Stanford Scientist Proving DNA Fragments In Vaccines Cause Autism.“It is possible that these contaminating fragments could be incorporated into a child’s genome and disrupt normal gene function, leading to autistic phenotypes.” Dr. Theresa Deisher, a PhD in Molecular and Cellular Physiology from Stanford University, the first person to discover adult cardiac derived stem cells, determined that residual human fetal DNA fragments in vaccines may be one of the causes of autism in children through vaccination.
Not only damaged human cells, but also healthy human cells can take up foreign DNA spontaneously. Foreign human DNA taken up by human cells will be transported into nuclei and be integrated into host genome, which will cause phenotype change. Hence, residual human fetal DNA fragments in vaccine can be one of causes of autism spectrum disorder in children through vaccination. Vaccine must be safe without any human DNA contaminations or reactivated viruses, and must be produced in ethically approved manufacturing processes.
A trio of recent publications in the journal NEURON reports the presence of hundreds of diverse de novo gene mutations indicating that autism spectrum disorder (ASD) may be a disease of genomic instability, with a significant environmental component. Altered double strand break formation and repair pathways (DSB) may be a commonality among the diverse genetic mutations that have been documented in ASD. US birth year change points in AD are apparent in 1980, 1988 and 1996, coinciding with the switch to or introduction of childhood vaccines contaminated with human endogenous retrovirus K (HERVK) and human fetal DNA fragments (6). We hypothesize that the HERVK and human fetal DNA contaminants could contribute to the genomic instability of ASD as demonstrated by de novo mutations.
Cell free DNA can be taken up by healthy cells via receptor mediated uptake or may spontaneously penetrate cell membranes that have altered permeability, for instance, during inflammatory reactions. Nuclear uptake of cell free DNA fragments is thought to provide a source for maintenance of DNA integrity during rescue of collapsed replication forks or base lesion repair. Spontaneous extracellular DNA uptake has also been exploited for gene therapy as well as for cellular gene correction (2,4,5,7,8, and 9). While free DNA uptake has been used advantageously, the process has also been associated with generation of mutations and chromosomal aberrations (3).
Vaccines manufactured using human fetal cells contain residual DNA fragments (50-500 bp) (Table I). It is possible that these contaminating fragments could be incorporated into a child’s genome and disrupt normal gene function, leading to autistic phenotypes. In this study we demonstrate foreign DNA uptake in human cells and genomic integration by incubating the cells with Cy3-labeled human Cot1 (placental) DNA fragments which represents contaminating residual human fetal DNA in vaccines.
Our measured genomic incorporation (0.003 to 0.04 pgs) of 0.2% – 0.6% of the whole genome in 24 to 48 hours seems high at first glance. However, our numbers are consistent with previous reports showing that exogenous DNA replaced up to 1% of the whole genome within 30 minutes (6). Although HL-60 cells did not spontaneously take up exogenous DNA in our experiments, the cell line has been used in the past as a model for spontaneous DNA uptake (8).
Cellular and nuclear DNA uptake in human foreskin fibroblast (HFF1) cells and in NCCIT cells suggests that embryonic and neonatal cell are more susceptible to DNA uptake than cells from a more mature source. These results indicate the need for further study of DNA incorporation from exogenous sources to compare the susceptibility of infants and toddlers versus teens and adults.
Increased DNA uptake after LPS activation suggests that systemic inflammation or immune responses could increase susceptibility for exogenous DNA uptake. Human diploid cell produced vaccines are contaminated by exogenous DNA fragments and a retrovirus, and vaccines elicit systemic inflammation and immune activation. Our future research goals are to localize the sites of DNA integration, to demonstrate phenotype changes caused by foreign DNA integration in factor dependent cell lines, and to determine the biological and/or pathological activities of Human Endogenous Retrovirus K (HERVK) fragments in vaccines.
Dr. Theresa Deisher, a PhD in Molecular and Cellular Physiology from Stanford University, the first person to discover adult cardiac derived stem cells, determined that residual human fetal DNA fragments in vaccines may be one of the causes of autism in children through vaccination.