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Archive for December, 2016

Cannabidiol Activates Neuronal Precursor Genes in Human Gingival Mesenchymal Stromal Cells.

https://www.ncbi.nlm.nih.gov/pubmed/27918106

Rajan TS, Giacoppo S, Scionti D Diomede F, Grassi G, Pollastro F, Piattelli A, Bramanti P Mazzon E, Trubiani O

In the last years, mesenchymal stromal cells (MSCs) from oral tissues have received considerable interest in regenerative medicine since they can be obtained with minimal invasive procedure and exhibit immunomodulatory properties. “From our results we hypothesize that human gingiva-derived MSCs conditioned with CBD could represent a valid method for improving the hGMSCs phenotype and thus might be a potential therapeutic tool in the treatment of neurodegenerative diseases.”

This study was aimed to investigate whether in vitro pre-treatment of MSCs obtained from human gingiva (hGMSCs) with Cannabidiol (CBD), a cannabinoid component produced by the plant Cannabis sativa, may promote human gingiva derived MSCs to differentiate towards neuronal precursor cells.

Specifically, we have treated the hGMSCs with CBD (5µM) for 24 h in order to evaluate theexpression of genes involved in cannabidiol signaling, cell proliferation, self-renewal and multipotency, and neural progenitor cells differentiation.

Next generation sequencing (NGS) demonstrated that CBD activates genes associated with G protein coupled receptor signaling in hGMSCs. Genes involved in DNA replication, cell cycle, proliferation and apoptosis were regulated. Moreover, genes associated with the biological process of neuronal progenitor cells (NCPs) proliferation, neuron differentiation, neurogenesis and nervous system development were significantly modulated.

From our results we hypothesize that human gingiva-derived MSCs conditioned with CBD could represent a valid method for improving the hGMSCs phenotype and thus might be a potential therapeutic tool in the treatment of neurodegenerative diseases.

https://www.ncbi.nlm.nih.gov/pubmed/27918106

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Fertilizer helps crops grow better, but nutrient runoff can lead to serious environmental problems. Ed Rosenthal, founder of Sarasota, Florida-based Florikan, had an idea to control the nutrient release to avoid runoff and maximize the benefit to the plant. He perfected the formula thanks to 40 hours of NASA consulting, and the fertilizer is now sold around the world—and also used on the ISS for project Veggie.“NASA’s expertise helped us advance our development by years. We were happy to pay it back.” — Ed Rosenthal, Florikan

https://spinoff.nasa.gov/Spinoff2017/index.html

https://spinoff.nasa.gov/Spinoff2017/ee_2.html

http://www.spacefoundation.org/programs/space-certification/certified-partners/certified-companies/florikan-esa-llc

(more…)

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A two-phase study evaluating the relationship between Thimerosal-containing vaccine administration and the risk for an autism spectrum disorder diagnosis in the United States ” the present study provides new epidemiological evidence supporting an association between increasing organic-Hg exposure from Thimerosal-containing childhood vaccines and the subsequent risk of an ASD diagnosis.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3878266/
David A Geier, Brian S Hooker,  Janet K Kern, Paul G King, Lisa K Sykes, and Mark R Geier

Results In phase I, it was observed that there was a significantly increased risk ratio for the incidence of ASD reported following the Thimerosal-containing DTaP vaccine in comparison to the Thimerosal-free DTaP vaccine. In phase II, it was observed that cases diagnosed with an ASD were significantly more likely than controls to receive increased organic-Hg from Thimerosal-containing hepatitis B vaccine administered within the first, second, and sixth month of life.

Conclusions: Routine childhood vaccination is an important public health tool to reduce the morbidity and mortality associated with infectious diseases, but the present study provides new epidemiological evidence supporting an association between increasing organic-Hg exposure from Thimerosal-containing childhood vaccines and the subsequent risk of an ASD diagnosis.

Autism spectrum disorder (ASD) is defined by standardized criteria of qualitative impairments in social interaction, qualitative impairments in communication, and restricted and stereotyped patterns of behavior, interests, and activities. A significant number of children diagnosed with ASD suffer a loss of previously-acquired skills, which is suggestive of neurodegeneration or a type of progressive encephalopathy with an etiological pathogenic basis occurring after birth. To date, the etiology of ASD remains under debate, however, many studies suggest toxicity, especially from mercury (Hg), in individuals diagnosed with an ASD. The present study evaluated concerns about the toxic effects of organic-Hg exposure from Thimerosal (49.55% Hg by weight) in childhood vaccines by conducting a two-phased (hypothesis generating/hypothesis testing) study with documented exposure to varying levels of Thimerosal from vaccinations. (more…)

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Autism: a novel form of mercury poisoning

https://www.ncbi.nlm.nih.gov/pubmed/11339848/

Autism: a novel form of mercury poisoning. Bernard S1, Enayati A, Redwood L, Roger H, Binstock T.
Autism is a syndrome characterized by impairments in social relatedness and communication, repetitive behaviors, abnormal movements, and sensory dysfunction

A review of medical literature and US government data suggests that: (i) many cases of idiopathic autism are induced by early mercury exposure from thimerosal which is found in vaccines given to young children  (more…)

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Our molecular profiling results show that NK603 and its isogenic control are not substantially equivalent. An integrated multi-omics analysis of the NK603 Roundup-tolerant GM maize reveals metabolism disturbances caused by the transformation process  http://www.nature.com/articles/srep37855

In conclusion, our integrative statistical and bioinformatics analysis allowed us to suggest a mechanistic link between the proteome and metabolome alterations observed and the insertion of a particular transgene. The transformation process and the resulting expression of a transgenic protein cause a general disturbance in the GM plant and it is clear that NK603 maize is markedly different from its non-GM isogenic line at the proteome and metabolome levels. In addition, our data correlates with previous studies, which observed higher amounts of ROS that act as free-radicals promoting oxidative stress in those transgenic plant materials. We also confirm a metabolic imbalance in energy and carbohydrate metabolism.

Robin Mesnage, Sarah Z. Agapito-Tenfen, Vinicius Vilperte, George Renney, Malcolm Ward, Gilles-Eric Séralini, Rubens O. Nodari & Michael N. Antoniou Scientific Reports 6, Article number: 37855 (2016)

Glyphosate tolerant genetically modified (GM) maize NK603 was assessed as ‘substantially equivalent’ to its isogenic counterpart by a nutrient composition analysis in order to be granted market approval. We have applied contemporary in depth molecular profiling methods of NK603 maize kernels (sprayed or unsprayed with Roundup) and the isogenic corn to reassess its substantial equivalence status. Proteome profiles of the maize kernels revealed alterations in the levels of enzymes of glycolysis and TCA cycle pathways, which were reflective of an imbalance in energy metabolism. Changes in proteins and metabolites of glutathione metabolism were indicative of increased oxidative stress. The most pronounced metabolome differences between NK603 and its isogenic counterpart consisted of an increase in polyamines including N-acetyl-cadaverine (2.9-fold), N-acetylputrescine (1.8-fold), putrescine (2.7-fold) and cadaverine (28-fold), which depending on context can be either protective or a cause of toxicity. Our molecular profiling results show that NK603 and its isogenic control are not substantially equivalent. (more…)

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Recurrent 6th nerve palsy in a child following different live attenuated vaccines: case report
Daryl R Cheng, Nigel W CrawfordEmail author, Michael Hayman, Christopher Buckley and Jim P Buttery
BMC Infectious Diseases http://bmcinfectdis.biomedcentral.com/articles/10.1186/1471-2334-12-105

Abstract Background
Recurrent benign 6th nerve palsy in the paediatric age group is uncommon, but has been described following viral and bacterial infections. It has also been temporally associated with immunization, but has not been previously described following two different live attenuated vaccines. There is limited information in the literature regarding the safety of a repeat dose of a live vaccine in this setting. Future immunizations should be considered on a case-by-case basis.

Case presentation
A case is presented of a 12 month old Caucasian boy with recurrent benign 6th nerve palsy following measles-mumps-rubella and varicella vaccines, given on separate occasions with complete recovery following each episode. No alternate underlying etiology was identified despite extensive investigations and review.

Conclusions
The majority of benign 6th nerve palsies do not have a sinister cause and have an excellent prognosis, with recovery expected in most cases. The exact pathophysiology is unknown, although hypotheses including autoimmune mechanisms and direct viral invasion could explain the pathophysiology behind immunization related nerve palsies. It is important to rule out other aetiologies with thorough history, physical examination and investigations. There is limited information in the literature regarding the safety of a repeat dose of a live vaccine in this setting. Future immunizations should be considered on a case-by-case basis. (more…)

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Phenethyl isothiocyanate: a comprehensive review of anti-cancer mechanisms.
Gupta P1, Wright SE2, Kim SH3, Srivastava SK4. https://www.ncbi.nlm.nih.gov/pubmed/25152445
Abstract
The epidemiological evidence suggests a strong inverse relationship between dietary intake of cruciferous vegetables and the incidence of cancer. Among other constituents of cruciferous vegetables, isothiocyanates (ITC) are the main bioactive chemicals present. Phenethyl isothiocyanate (PEITC) is present as gluconasturtiin in many cruciferous vegetables with remarkable anti-cancer effects. PEITC is known to not only prevent the initiation phase of carcinogenesis process but also to inhibit the progression of tumorigenesis. PEITC targets multiple proteins to suppress various cancer-promoting mechanisms such as cell proliferation, progression and metastasis. Pre-clinical evidence suggests that combination of PEITC with conventional anti-cancer agents is also highly effective in improving overall efficacy. Based on accumulating evidence, PEITC appears to be a promising agent for cancer therapy and is already under clinical trials for leukemia and lung cancer. This is the first review which provides a comprehensive analysis of known targets and mechanisms along with a critical evaluation of PEITC as a future anti-cancer agent.
Copyright © 2014 Elsevier B.V. All rights reserved.

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